Group leader: Klaas J. Hellingwerf

Group name: Molecular Microbial Physiology

Other group staff members: Crielaard, Teixeira de Mattos, Klis

Location: Swammerdam Institute for Life Sciences, BCA, University of Amsterdam

Web page: http://bioicrs6.chem.uva.nl/microbio/index.html, http://www.science.uva.nl//sils/

E-Mail: khelling@science.uva.nl

Phone: +31 20 5257055

 

Relevant research interests:

In the research group Molecular Microbial Physiology (SILS, UvA; staff members: Crielaard, Teixeira de Mattos, Klis and Hellingwerf) the central research topic is the molecular basis of adaptation of microorganisms to (stress) signals from their environment. These studies cover the (sub)molecular and (inter)cellular levels. As stress signals we use e.g. (UV) light, high-temperature, weak acid preservatives, nutrient limitation, etc. Part of this work is very chemically (i.e. molecularly) oriented in the sense that we try to understand the rearrangements within molecules that are required for these responses. At the other extreme we try to understand how this responsiveness helps organisms to increase their evolutionary competitiveness in the environment.

 

Current system biology activities:

At the central level in our research, therefore, we try to generate an overview of the mechanisms and pathways that are available for responses and adaptation, in a limited number of strategically chosen model organisms (like Escherichia coli, Bacillus subtilis and Saccharomyces cerevisiae). In addition to that, we want to understand how these pathways interact, to jointly generate the phenotype of an organism, as it is observable in a model system like the chemostat and/or in the natural environment. In other words, in this part of our research program we try to understand the rationale of why physiology and adaptation is organized the way it is. We consider this the heart of ‘systems biology’.

            On basis of the info above my estimate is that more than half of our efforts are within the area of ‘systems biology’. We tackle the challenges and problems in this area with the tools of biochemistry, physiology, molecular genetics and bioinformatics. I think that additional expertise at the level of mathematics and statistical physics is required to significantly increase the quality of future work in this field. The work of Uri Alon (Weizmann Institute) is a good example of how and in which direction this field is, and will be further, progressing.

Relevant collaborations (e.g.):

 

Representative publications (possibly links to pdf files):