Group name: Section of Biomedical NMR, Department of Biomedical Engineering
Location: Eindhoven University of Technology
Web page: <http://www.bmi2.bmt.tue.nl/nmr/>
E-Mail: <mailto:k.nicolay@tue.nl>
Phone: +31 40 247 5789 / 247 3787
Relevant research interests:
- The regulation of the ATP synthesizing activity of the mitochondrion in skeletal and cardiac muscle in health and disease (diabetes, critical illness, genetic disorders);
- The relation between muscle phenotype (fast-twitch, slow-twitch) and mitochondrial characteristics;
- The use of non-invasive NMR techniques to measure bio-energetic properties, and in particular mitochondrial activity, in muscle in animal models and man;
- The integration of in vitro studies on isolated mitochondria, skinned muscle fibers and excised muscles with in vivo studies to determine mass flow and concentration control characteristics at different levels of eukaryotic system organization. The combination of molecular biological (transgenic mice), biophysical (NMR) and modelling techniques to understand adaptive changes in mitochondrial control characteristics in response to gain or loss of enzyme function.
Current system biology activities:
- Biochemical, biophysical and physiological studies of skeletal and cardiac muscle of mice deficient in creatine kinase iso-enzymes. Experimentation and modelling are combined at several in vitro and in vivo levels of biocomplexity to learn to understand alterations in mitochondrial and muscle phenotype.
- Combined animal model and human studies to measure, understand and therapeutically alter mitochondrial and phenotype muscle in control and diseased states.
Relevant collaborations:
- National: - Jeneson (UU)
- Wagenmakers,
Van der Vusse (UM)
- International: - De
Graaf, Rothman (Yale)
- Walliman (ETH)
- Wilson (MSU)
- Gellerich (Halle)